Translational_Unit

Part:BBa_K3521005:Design

Designed by: Kong Yangyang   Group: iGEM20_SHSID   (2020-10-14)


pT7-FnCpf1-His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1784
    Illegal NheI site found at 3504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mutated C to A to remove SapI restriction site to meet the type IIS standard.

Source

The sequence of FnCpf1 comes from the genomic sequence of Francisella novicida.

References